-Introduction: in vivo and in vitro studies.
-Physiological solution
-Cell cultures:
cells separation; counting cells;
cryo-conservation;
transfection.
-Molecular biology:
Introduction
DNA, RNA purification
Agarose gels
restriction enzymes
ligase
DNA vectors
DNA sequencing
RT
PCR
Real Time PCR
Gene expression
Gene silencing techniques
Mutagenesis
Gene promoter studies
Transfections
iRNA and microRNA.
-Proteins:
purification
- Gel Electrophoresis-Protein-protein interactions
-DNA-protein interactions.
-Microarray
-Proteomic array
-Bioinformatics