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BLOOD ANALYSES: LABORATORY TECHNIQUES AND INTERPRETATION OF THE RESULTS Single discipline educational activity
Course Sheet Academic Year of enrolment:
Professor and Collaborators:
Minimum hours of attendance:
Hours of classroom activity:
Prerequisites:
Knowledge of the cell organization, of the biological molecules classes and characteristics. To have passed the Animal Biology and Plant Biology exam and Biochemistry exam.
Objectives
Contents Blood: analysis of the acqueous matrix and of the cellular part.
Plasma
Cellular elements: erythrocytes, leukocytes and trombocytes.
Laboratory tecniques applied to blood analyses.
Blood smear.
Serum protein electrophoresis.
Flow cytometry.
Immunochemistry tecniques.
Molecular diagnostics.
Extended Syllabus Blood: analysis of the acqueous matrix and of the cellular part.
Plasma: plasmatic proteins, albumins, immunoglobulins, blood levels of organic and inorganic components.
Cellular elements: haematocrit, cell count and blood cytotypes distinction.
Erythrocytes: haemoglobin, blood group, evaluation of the erythrocytes parameters.
Leukocytes: classification and leukocytes count.
Trombocytes: coagulation events, thrombocytes count and parameters.
Laboratory tecniques applied to blood analyses.
Preparation, staining, observation and results’ interpretation of a blood smear.
Serum protein electrophoresis: baseline principle, sample preparation, protein running and blot transfer.
Flow cytometry: baseline principles, distinction of leukocytes populations based on morphology and surface markers. Clinical applications: blood count, reticolocytes, leukemia, CD4, HLA.
Immunochemistry methods for blood components quantification: RIA, ELISA. Spettrometry
Molecular diagnostics: extraction and analysis of nucleic acids. PCR. Tumoral markers.
Recommended Bibliography The required material will be provided by the teachers.
Methods of Provision
Teaching Methods Theoretical lessons (power point presentations) followed by laboratory practice activies.
Evaluation methods Verification of learning:
Oral exam on at least three different topics.
There is no grade. The exam will be considered passed if the student will show an adequate level of knowledge for all the arguments of the exam questions.
Contacts/More Information Office hours for students:
Mon-Friday, by appointment